competitive elisa analysis  (Biostatistical Consulting)

Journal: Frontiers in Immunology

Article Title: A Telomerase-Derived Peptide Exerts an Anti-Hepatitis B Virus Effect via Mitochondrial DNA Stress-Dependent Type I Interferon Production

doi: 10.3389/fimmu.2020.00652

Figure Lengend Snippet: The anti-HBV effect of GV1001 on human hepatocytes. (A) HBsAg and extracellular HBV DNA levels were evaluated. PBS (0.5%), GV1001 (10 μM), and LMV (10 μM) were added to HepG2 cells and Huh-7 cells transiently transfected with the pHBV-1.2X-wild-type plasmid. (B) Cell viability assays (MTS) were conducted. (C) Quantitative PCR was performed on the supernatant of HepG2 cells transfected with 1.2x-WT plasmid and treated with GV1001 at different doses. The IC50 was calculated. (D,E) Extracellular HBV virion and HBeAg levels were measured using qPCR and ELISAs from the supernatants of HepG2-2.15 cells after the administration of phosphate-buffered saline and GV1001 for 48 h. (F) A lactate dehydrogenase assay was carried out on HepG2-2.15 and HepG2 cells to determine the cytotoxicity induced by GV1001. Data represent the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 versus PBS.

Article Snippet: For the detection of 8-hydroxy-2′-deoxyguanosine (8-OHdG) activity, competitive ELISAs from an 8-OHdG analysis kit (OxiSelect Oxidative DNA Damage ELISA kit, Cell Biolabs, San Diego, CA, United States) was used according to the manufacturer’s protocol.

Techniques: Transfection, Plasmid Preparation, Real-time Polymerase Chain Reaction, Saline, Lactate Dehydrogenase Assay

Journal: Frontiers in Immunology

Article Title: A Telomerase-Derived Peptide Exerts an Anti-Hepatitis B Virus Effect via Mitochondrial DNA Stress-Dependent Type I Interferon Production

doi: 10.3389/fimmu.2020.00652

Figure Lengend Snippet: Anti-HBV effect of GV1001 on a transgenic mouse model. (A) Female transgenic mice were injected with phosphate-buffered saline, lamivudine, (0.5 mg/kg) or GV1001 (0.05 mg/kg), twice weekly. At 4 and at 8 weeks later, HBsAg from mouse serum was measured by ELISAs. (B) After the extraction of viral DNA from serum, HBV DNA was quantified by qPCR. This study was conducted in accordance with the guidelines established by the Seoul National University Institutional Animal Care and Use Committee (Approval No. SNU-111025-6-3). Data represent the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 versus PBS.

Article Snippet: For the detection of 8-hydroxy-2′-deoxyguanosine (8-OHdG) activity, competitive ELISAs from an 8-OHdG analysis kit (OxiSelect Oxidative DNA Damage ELISA kit, Cell Biolabs, San Diego, CA, United States) was used according to the manufacturer’s protocol.

Techniques: Transgenic Assay, Injection, Saline, Extraction